FIG. 5.

Trichamber compartment culture system. The experimental apparatus used to determine the effects of Us3 null on retrograde and anterograde transport within axons has been previously described (11). For axon-mediated infection of neuron cell bodies (A), infection was initiated in the N compartment. As infection proceeded, cell bodies and extracellular medium from the S compartment were collected and titered. The middle compartment (M) was filled with media and methocel to act as a diffusion barrier. For determination of the neuron-to-cell spread of infection (B), detector PK15 cells were plated in the N compartment after axons had penetrated the N compartment. Infection was initiated in the S compartment, and after 24 h, the infection spread from axons to PK15 cells. Medium from the N compartment was harvested and titered.