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. 2006 Jul;80(13):6368–6377. doi: 10.1128/JVI.00211-06

FIG. 1.

FIG. 1.

Replication and transcription activities of PeGFP fusion protein. (A) Domain organization of P protein, showing domains I, II, and III and the hinge region. The site of eGFP incorporation at aa position 196 is indicated by a vertical dotted line. (B) Expression of PeGFP fusion protein in transfected cells. Cells transfected with plasmids encoding P (lanes 2 and 5) or PeGFP (lanes 3 and 6) proteins or no plasmid (lanes 1 and 4) were radiolabeled with Expre35S35S label. The radiolabeled proteins were immunoprecipitated with antibodies as shown on the top (α-P, anti-P; α-eGFP, anti-eGFP), analyzed by SDS-PAGE, and detected by fluorography. Size markers in kDa are shown on the left. P and PeGFP proteins are identified on the right. (C) Replication and transcription activities of PeGFP protein relative to Pwt as determined by DI-particle replication or minigenome transcription assays (20, 40). The histograms represent the average data from three independent experiments, with standard deviations shown by error bars. Repln, replication; Txn, transcription.