FIG. 1.

CVB3 genome organization and plasmid reporter constructs. (A) Schematic representation of the CVB3 genome. Shown is the positive-sense viral RNA containing a single open reading frame, from the product of which viral proteins are processed co- and posttranslationally. The 5′ UTR contains the internal ribosome entry site (IRES) used to direct translation of the marker proteins shown in panel B. (B) Six dicistronic plasmid constructs were prepared to examine the effects of viral proteins on protein trafficking. Two reporter proteins were employed, placental SEAP and eGFP(mem), which translocates from the cytoplasm to the plasma membrane; for each reporter, three viral proteins were cloned upstream. Two control constructs were also prepared (not shown); these expressed the reporter proteins but no viral gene.