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. 2006 Jul;80(13):6637–6647. doi: 10.1128/JVI.02572-05

FIG. 7.

FIG. 7.

3A localizes to the Golgi early in CVB3 infection, and trafficking proteins ultimately accumulate in the ER. (A) Cells were infected with wild-type (WT) CVB3 at an MOI of 0.1, and at early (3 h; top row) or late (5 h; bottom row) time points postinfection, the cells were fixed and stained with anti-3A serum (red fluorescence), Hoechst 33342, and GM130 antibody (far-red fluorescence). GM130 was pseudocolored green in the merged images (last column). (B) A DsRed(mem)-expressing recombinant CVB3 was used to infect HeLa (RW) cells at an MOI of 0.1. At an early time point (3 h; top row) and a later time point (5 h; bottom row), cells were fixed and stained with Hoechst 33342, anti-GM130 (conjugated to fluorescein isothiocyanate, green fluorescence), and anticalnexin (far-red fluorescence) to examine the localization of transiting DsRed(mem) protein. Calnexin was pseudocolored green in the merged images. DsRed(mem) colocalization with Golgi or ER is shown in white pixels.