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Binding of TRIM chimeras to HIV-1 CA-NC complexes. Hypotonic lysates of HeLa cells expressing different TRIM chimeric proteins were incubated with the in vitro-assembled HIV-1 CA-NC complexes. The total input (Input) from the lysate and the pellet (Pellet) from the sucrose gradient were tested for the presence of TRIM proteins which are C terminally tagged with HA. The bottom panel shows the amount of HIV-1 CA-NC protein pelleted through the 70% sucrose cushion.
