TABLE 1.
Summary of HIV-1 infection in cells expressing TRIM proteins and chimeras
| TRIM variant | HIV-1 infectiona
|
||
|---|---|---|---|
| X = 6 | X = 34 | X = 21 | |
| TRIMX | +++ | ++++ | +++ |
| TRIMX-5(B30.2) | + | +++ | ++++ |
| TRIMX-5(L2-B30.2) | ++++ | + | ++++ |
| TRIMX-5(CC-B30.2) | + | + | ++++ |
| TRIMX-5(B-box-B30.2) | NDb | ND | ++ |
| TRIMX-5(L1-B30.2) | ND | ND | + |
| TRIM5-X(B-box 2) | ND | ND | +++ |
a
Following incubation with HIV-1-GFP, the level of infection in cells expressing the indicated TRIM proteins was assessed by measuring GFP-positive cells. A level of infection comparable to that seen in cells transduced with the empty LPCX vector is designated +++. Levels of infection that range from 30 to 60% of the level observed in the LPCX-transduced control cells are designated ++. Levels of infection that range from 1 to 29% of the level in LPCX-transduced cells are designated +. The designation ++++ indicates a level of infection greater than 120% of the level in LPCX-transduced cells.
b
ND, not determined.