FIG. 6.

Impairment of cleavage of concatemeric DNA in the absence of PrV UL25. RK13 or RK13-UL25 cells were infected with PrV-Ka, PrV-ΔUL17, or PrV-ΔUL25F at an MOI of 5 and harvested at 16 h p.i. Whole-cell DNA was isolated, digested with BamHI, separated on a 0.8% agarose gel, and blotted onto a nylon membrane. Parallel blots were probed with labeled genome-end-specific BamHI fragments 13 (A) and 14′ (B). Bound radioactivity was recorded by a phosphorimager and quantified. Values were calculated with respect to bound radioactivity present on BamHI fragment 8′, set as 100% for each lane. Identification of fragments and their sizes are indicated. “Junction” refers to the junction fragment derived from head-to-tail-fused termini.