FIG. 5.

(A) CHV1-p29 hydrophobicity plot. Hydrophobicity was calculated by using the algorithm of Kyte-Doolittle with a window size of 7 amino acids using the SDSC Biology workbench web-based tool (34). (B) Extraction and Western blot analysis of p29 in CHVI-infected strain EP802. The P90,000 fraction was extracted with either 1 M NaCl, 0.1 M Na₂CO₃ (pH 11.5), or 2 M urea and subjected to centrifugation at 90,000 × g, yielding the soluble fraction (S) and pellet (P). The total P90,000 fraction was also fractionated into aqueous (AP) and detergent-soluble (DP) phases after treatment with 1% Triton X-114. Samples were analyzed by Western blotting using p29 antibodies.