FIG. 2.

Subcellular localization of sites of synthesis of individual HDV RNA strands. Huh7 cells were cotransfected with BrUTP, HDAg mRNA and either genomic RNA (G+m) or antigenomic RNA (AG+m) as previously described (25, 26). Cells were treated with actinomycin D (AMD) (+) and with (+) or without (−) α-amanitin before transfection. Transfection of genomic (G), antigenomic (AG), or mRNA (m) alone, none of which alone lead to RNA replication, served as negative controls. At 15 min after RNA transfection, BrUTP-labeled RNA was detected by staining with BrdU-specific antibody. BrUTP labeling, Cy2 (green); C23, Texas Red (red).