FIG. 3.

(A) HDV genomic RNA synthesis is colocalized with the PML body. The BrUTP-labeled RNA and PML body are identified by red (Cy3) and green (FITC) staining, respectively. The arrows indicate the convergence of PML body and BrUTP labeling, and the asterisks indicate the BrUTP labeling in the nucleolus-like structures. (B) HDAg is colocalized with HDV genomic and antigenomic RNA synthesis. The BrUTP labeling and HDAg are identified as red (Cy3) and green (FITC), respectively. The RNA transfection and drug treatments were the same as described for Fig. 2. The incubation time was 60 min after RNA transfection. AMD, actinomycin D.