FIG. 4.

Colocalization of HDAg and PML (A) or SC35 (B). Huh7 cells were cotransfected with HDAg-encoding mRNA and either genomic RNA (G+m) or antigenomic RNA (AG+m) as indicated. At 60 min after RNA transfection, the cells were examined for the distribution of HDAg and PML or SC35 by immunostaining. Untransfected Huh7 cells served as negative control (−). The cells were treated with actinomycin D (AMD) at 5 μg/ml prior to transfection. PML and SC35 are identified by Cy2 (green), and HDAg is identified by Cy3 (red). The arrowheads show the colocalization of HDAg and PML or SC35.