TABLE 2.

Predicted TCR beta chain CDR3 region amino acid sequences of FYT A24 CD8⁺ cell lines and clones from individuals O9, O22, and RV1

Gene segment	Amino acid at CDR3 positiona:
	95	96	97	98	99	100	101	102	103
O9 cell line pool	T	P	S	G	G	Y	N	E	Q
O9 cell lines
A 14/30	T	P	S	G	G	Y	N	E	Q
B 4/30	S	S	A	G	G	Y	N	E	Q
C 3/30	S	L	S	G	A	Y	N	E	Q
D 1/30	S	L	S	G	G	Y	N	E	Q
E 1/30	S	L	S	G	G	Y	N	E	Q
F 1/30	S	F	A	G	G	Y	N	E	Q
G 1/30	S	F	S	G	G	Y	N	E	Q
H 1/30	T	P	G	G	G	Y	N	E	Q
I 1/30	N	L	A	G	G	Y	N	E	Q
J 1/30	S	L	A	G	G	F	T	E	A
K 1/30	T	F	S	G	A	Y	N	E	Q
L 1/30	S	S	G	G	G	Y	N	E	Q
O9 clone 1	S	S	G	G	G	Y	N	E	Q
O9 clone 2	T	L	G	G	A	Y	N	E	Q
O22 clone 1	S	S	A	G	G	Y	N	E	Q
O22 clone 2	S	S	A	G	G	Y	N	E	Q
O22 clone 3	S	S	A	G	G	Y	N	E	Q
O22 clone 4	S	S	A	G	G	Y	N	E	Q
O22 clone 5	S	S	A	G	G	Y	N	E	Q
O22 clone 6	S	S	A	G	G	Y	N	E	Q
O22 clone 7	S	S	A	G	G	Y	N	E	Q
RV1 cell line pool	S	S	A	G	G	Y	N	E	Q

^aFor the O9 cell line, both a pool sequence and molecular clones were obtained. Letters in bold indicate amino acids that are probably contributed by the BV5.1 gene segment, and underlining indicates amino acids that are probably contributed by the BJ2.1 gene segment. The TCR beta CDR3 loop is predicted to span amino acids 95 to 106, with a framework cysteine at position 92.