FIG. 5.

Classification of viral genes required for inhibition of STAT1 phosphorylation by PRV Be. (A) The indicated cells were pretreated with CHX (100 μg/ml) or PAA (400 μg/ml) for 30 min before being mock infected or infected with PRV Be at a high MOI for 9 h prior to stimulation with IFN-β for 30 min. CHX or PAA was maintained in the cell medium throughout the infection and IFN stimulation. (B) Cells were infected with PRV EP0-1 or PRV Ba for 9 h prior to stimulation with IFN-β for 30 min. Representative immunofluorescent images stained with anti-pSTAT1 and anti-gB antibodies and Hoechst DNA stain are shown. Cells outlined in the merged panels are magnified in the inset column. Image acquisition settings were kept constant for all panels.