TABLE 2.
Host genes induced threefold or more 12 h postinfection with PRV Be and/or treatment with IFNβa
| Accession no. | Gene | Avg increase (n-fold)a
|
||
|---|---|---|---|---|
| IFN | IFN + PRV Be | PRV Be | ||
| Threefold (or more) increase in IFN | ||||
| X52713 | Mx3 | 267.7 | − | − |
| AF068268 | 2′-5′ oligoadenylate synthetase 2 | 42.4 | − | − |
| AA892553 | STAT1 | 31.9 | − | − |
| AA799569 | EST (BLAST similarity to STAT2) | 22.1 | − | − |
| U16025 | MHC class Ib RT1-M3 | 19.2 | − | − |
| U72741 | Beta-galactoside binding lectin | 17.4 | − | − |
| D10729 | Proteasome subunit beta type 8 precursor | 15.0 | − | − |
| D88250 | Serine protease homologous to complement C1s precursor | 14.8 | − | − |
| AF065438 | Galactoside-binding lectin | 10.8 | − | − |
| D45250 | Proteasome activator rPA28 beta subunit | 9.8 | − | − |
| AA926129 | Manganese-containing superoxide dismutase | 8.6 | − | − |
| X76453 | HRAS-like suppressor (H-rev107) | 7.3 | − | − |
| AA799803 | Serine protease homologous to complement C1r precursor | 7.0 | − | − |
| S81497 | Lysosomal acid lipase A | 6.6 | − | − |
| AF025308 | MHC class Ia RT1-A2 | 6.6 | − | − |
| AI177256 | EST | 6.1 | − | − |
| M10094 | MHC class I truncated cell surface antigen | 5.9 | − | − |
| M31018 | MHC class I alpha chain precursor | 5.3 | − | − |
| D45249 | Proteasome activator rPA28 subunit alpha | 4.9 | − | − |
| AF036537 | Receptor-interacting serine-threonine kinase 3 | 4.9 | − | − |
| M24026 | MHC class I RT1 (RT44), u haplotype | 4.8 | − | − |
| AI232284 | EST (BLAST similarity to MHC class I) | 4.6 | − | − |
| M11071 | MHC class I RT1.Aw3 | 4.3 | − | − |
| AF074609 | MHC class I RT1-EC3 | 4.1 | − | − |
| AF017437 | CD47 antigen (integrin-associated signal transducer) | 3.9 | − | − |
| U18942 | dsRNA-specific adenosine deaminase | 3.8 | − | − |
| AF074608 | MHC class Ib RT1-EC2 | 3.8 | − | − |
| AI176710 | Neuron-derived orphan nuclear receptor 1/2 | 3.5 | − | 6.6 |
| L16532 | 2′,3′-cyclic nucleotide 3′-phosphodiesterase | 3.4 | − | − |
| AF054826 | Vesicle-associated membrane protein 5 (VAMP5) | 3.4 | − | − |
| L40362 | MHC class I RT1.C type | 3.3 | − | − |
| AA875037 | Serine proteinase inhibitor mBM2A | 3.2 | − | − |
| U53475 | RAB8B GTPase, member RAS oncogene family | 3.2 | − | − |
| AA893280 | Adipose differentiation-related protein | 3.1 | − | − |
| L25387 | Phosphofructokinase C | 3.1 | − | − |
| AA892250 | Kainate receptor | 3.0 | − | − |
| U09870 | Major vault protein (MVP) | 3.0 | − | − |
| Threefold (or more) increase in both IFN and IFN + PRV Be | ||||
| M80367 | IFN-induced guanylate-binding protein 2 (p67) | 273.7 | 35.4 | − |
| U17035 | Small inducible cytokine B subfamily (C-X-C) member 10 | 245.1 | 242.9 | 79.7 |
| AA799861 | IFN regulatory factor 7 (moderately similar) | 229.4 | 520.1 | 17.7 |
| Y07704 | Best5/viperin | 228.3 | 257.4 | 12.6 |
| X52711 | Mx1 | 173.3 | 18.2 | − |
| AA891944 | EST (BLAST similarity to Mx2) | 111.2 | 26.3 | − |
| D10757 | Proteasome subunit R-RING12 | 62.6 | 7.7 | − |
| AI235890 | MHC class Ib RT1-BM1, alpha chain | 57.5 | 8.4 | − |
| X57523 | Tap1/antigen peptide transporter | 27.8 | 6.8 | − |
| AI013987 | PKR | 18.9 | 13.2 | 3.5 |
| L23128 | MHC class Ib RT1, H2-TL-like | 16.0 | 6.9 | − |
| Z18877 | 2′-5′ oligoadenylate synthetase 1 | 9.9 | 3.9 | − |
| M34253 | IFN regulatory factor 1 (IRF1) | 8.4 | 4.3 | − |
| L40364 | MHC class Ib RT1-CE12 | 7.2 | 3.8 | − |
| M24067 | Plasminogen activator inhibitor 1 (PAI-1) | 5.9 | 7.3 | 3.2 |
| L01624 | Serum/glucocorticoid-regulated kinase | 5.2 | 25.0 | 9.1 |
| AI170268 | Beta-2-microglobulin | 5.2 | 6.9 | − |
| M24324 | MHC class I CE1 | 4.9 | 3.6 | − |
| X61381 | Interferon-induced transmembrane protein 3 | 4.7 | 5.0 | − |
| AI176456 | EST (BLAST similarity to MHC) | 4.3 | 15.0 | 3.5 |
| AF029240 | MHC class Ib RT1-S3 | 4.0 | 3.4 | − |
| Threefold (or more) increase in IFN + PRV Be | ||||
| M11794 | Metallothionein | − | 36.3 | 4.2 |
| S67722 | Cyclooxygenase-2 (COX-2) | − | 19.2 | 17.5 |
| AI102562 | EST (BLAST similarity to metallothionein) | − | 12.3 | − |
| AI137583 | Inhibitor of DNA binding 2 | − | 11.8 | 7.2 |
| U09401 | Tenascin C | − | 11.7 | 3.4 |
| U48592 | Interleukin-1 receptor accessory protein | − | 10.3 | − |
| L11035 | T-cell receptor alpha chain | − | 8.7 | 10.3 |
| AA891576 | Complement component 1, q subcomponent | − | 8.5 | 9.1 |
| M64711 | Endothelin 1 | − | 7.7 | − |
| AF000942 | Inhibitor of DNA binding 3 | − | 6.3 | − |
| L14937 | Proprotein convertase subtilisin/kexin type 4 | − | 6.3 | 6.4 |
| U60977 | Lipid raft protein flotillin 1 | − | 5.9 | − |
| AA891499 | Predicted: similar to RIKEN | − | 5.9 | 5.2 |
| X80395 | Vesicular acetylcholine transporter (VAChT) | − | 5.4 | 4.5 |
| S98336 | Mullerian-inhibiting factor precursor | − | 5.2 | 4.8 |
| AA859519 | Predicted: similar to RNA binding protein gene | − | 4.9 | 4.0 |
| X07320 | Phosphorylase b kinase, gamma subunit | − | 4.7 | 3.8 |
| S77556 | Discoidin domain receptor family, member 1 | − | 4.7 | 4.7 |
| M61875 | CD44 antigen, transmembrane glycoprotein | − | 4.7 | 5.2 |
| X91988 | STAT5b | − | 4.6 | 3.8 |
| AA944156 | B-cell translocation gene 2 | − | 4.5 | 3.7 |
| AA892333 | Predicted: tubulin, alpha 6 | − | 4.4 | 6.1 |
| AA800613 | Zinc finger protein 36 | − | 4.4 | − |
| AI169756 | Mitogen-inducible gene 6 | − | 4.3 | 4.1 |
| AA875509 | Mdm2 | − | 4.3 | − |
| L16764 | Heat shock protein 70 | − | 4.3 | − |
| L01267 | Transcription initiation factor IIF beta subunit | − | 4.2 | − |
| AA799406 | EST | − | 4.2 | 4.1 |
| X62875 | High-mobility group protein Y | − | 4.1 | − |
| M33312 | Cytochrome P450 | − | 4.0 | − |
| X06769 | c-fos | − | 3.8 | − |
| M32061 | Alpha-2B-adrenergic receptor | − | 3.8 | − |
| AF087944 | Monocyte differentiation antigen CD14 precursor | − | 3.7 | 3.3 |
| AA891751 | EST (BLAST similarity to voltage-gated sodium channel) | − | 3.7 | − |
| U09361 | Tenascin C | − | 3.7 | − |
| M63282 | Activating transcription factor 3 | − | 3.7 | − |
| AI232783 | Glutamine synthetase 1 | − | 3.7 | − |
| L35558 | Neuronal/epithelial high-affinity glutamate transporter | − | 3.5 | 3.8 |
| AF030358 | Fractalkine precursor (C-X3-C) motif | − | 3.5 | 9.8 |
| M35270 | Alanine-glyoxylate aminotransferase | − | 3.4 | 3.2 |
| AI169327 | Tissue inhibitor of metalloproteinase 1 | − | 3.4 | − |
| AF061971 | Palmitoyl-protein thioesterase 2 | − | 3.3 | − |
| AF001417 | Kruppel-like transcription factor | − | 3.1 | − |
| AI230260 | Casein kinase 2 | − | 3.1 | − |
| U19866 | Activity-regulated cytoskeletal-associated protein (Arc) | − | 3.1 | − |
| AF020618 | Myd116/protein phosphatase regulatory subunit | − | 3.0 | − |
a
The average increase compared to mock-treated cells is indicated for each experimental condition. −, not significantly different or absent.