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Validation of microarray data by real-time PCR
| Gene | Condition | Increase (n-fold)
|
|
|---|---|---|---|
| Microarray | RT-PCR | ||
| PKR | IFN, 3 h | 37.0 | 0.4 |
| IFN + PRV Be, 3 h | 37.2 | 13.9 | |
| Be, 3 h | 1.1 | 1.3 | |
| IFN + PRV Be, 12 h | 13.2 | 0.7 | |
| Be, 12 h | 3.5 | 0.2 | |
| Mx1 | IFN, 3 h | 95.4 | 20.0 |
| IFN + PRV Be, 3 h | 60.6 | 3,880.0 | |
| Be, 3 h | 1.6 | 11.2 | |
| IFN, 12 h | 173.3 | 260,000.0 | |
| IFN + PRV Be, 12 h | 18.2 | 2,600.0 | |
| Be, 12 h | 1.8 | 11.0 | |
