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. 2006 Jul;80(13):6324–6332. doi: 10.1128/JVI.02328-05

FIG. 2.

FIG. 2.

Macrophage tropism of HIV-1 R5 envelopes amplified from patient tissues. Amplified envelopes were cloned into pSVIIIenv and expressed on HIV-1 particles. Infectivity was titrated on HeLa TZM-BL cells (HeLa/CD4/CCR5; JC53 clone [29]) and on primary macrophages. Infectivity was assessed by immunostaining in situ and estimating the number of infected cells as described previously (27). Macrophage infectivity is plotted as a percentage of that recorded on HeLa TZM-BL cells. Light symbols represent envelopes that failed to confer any macrophage infectivity, i.e., macrophage infectivity was less than the value shown.