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Strong silencing of KIF14 eliminates midbody concentration of KIF14 but does not prevent chromosome segregation, progression to telophase, or furrow ingression. Immunofluorescence microscopy was used to evaluate chromosome segregation and furrow ingression in KIF14 siRNA-transfected HeLa cells. DNA staining (Hoechst), blue channel; KIF14 localization, green channel; α-tubulin localization, red channel. A, anaphase, T, telophase, C, cytokinesis.
