TABLE 2.

Penetrance of apoptotic phenotypes associated with moderate KIF14 silencing^a

Cell cycle stageb	% Penetrance of:
	Luciferase	M-1
Interphase	3	21
Metaphase	0	28
Cytokinesis	0	8
Postbinucleation	0	42

^aTime-lapse microscopy was utilized to determine the percentage of penetrance. HeLa cells were transfected with either a luciferase control siRNA or the moderate KIF14 siRNA M-1. Time-lapse was initiated 30 h posttransfection and was terminated 24 h later. The baseline level of apoptosis was determined by counting the number of apoptotic events that occurred within a population of 100 luciferase siRNA-transfected cells. For M-1 transfected cells, 75 apoptotic events were scored.

^bAll apoptotic events were segregated based upon the stage of the cell cycle where apoptosis occurred.