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Nickel ions suppressed the expression of the gpt transgene in G12 cells. (a) G12 cells were exposed to various concentrations of NiCl2 for 24 h. (b) G12 cells were exposed to 100 μM NiCl2 for selected time intervals. Total RNA was isolated, and the expression of the gpt transgene was examined using Northern blotting. The ethidium bromide staining of total RNA was performed to assess the RNA loading.
