FIG. 6.

NPM and rpL5 colocalize with nuclear and cytosolic ribosome subunits. HeLa cells transduced with vector (top panels) or His-NPMdL (middle panels) or treated with LMB (bottom panels) were divided into cytoplasmic and nuclear fractions and subjected to sucrose gradient centrifugation. Absorbance was monitored at 254 nm, and fractions containing 40S, 60S, 80S, and polysome units were collected. Proteins from each fraction were separated by SDS-PAGE, transferred to PVDF membranes, and immunoblotted with antibodies recognizing NPM, the His epitope, and rpL5. α, anti.