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. 2000 May;11(5):1875–1886. doi: 10.1091/mbc.11.5.1875

Figure 3.

Figure 3

Histology of heart sections of wild-type and Smg GDS−/− mice and enhanced apoptosis in the Smg GDS−/− heart. (Aa and Ba) Representative sagittal sections (20×) of the wild-type heart (Aa) and the Smg GDS−/− heart (Ba) stained with HE at 1 d after birth. The Smg GDS−/− heart was dilated and expanded with thinning of atrial, ventricular, and septal walls compared with the littermate control. (Ab and Bb) Left ventricular walls (boxed regions in Aa and Ba) of the wild-type heart (Ab) and the Smg GDS−/− heart (Bb) at high-power (200×) magnification. Cellularity of the cardiomyocyte was markedly reduced in the Smg GDS−/− heart. (Ca and Da) TUNEL staining of the Smg GDS+/− heart and the Smg GDS−/− heart. They were prepared from littermate mice. The Smg GDS−/− heart (20×; Da) at the day of birth showed conserved architecture compared with the wild-type heart (20×; Ca). Cellularity of Smg GDS−/− cardiomyocytes was not reduced in contrast to marked hypocellularity of cardiomyocytes at 1 d after birth, as shown in Ba and Bb. (Cb and Db) Middle-power (100×) magnification of the right ventricles of the Smg GDS+/− heart (Cb; boxed in Ca) and the Smg GDS−/− heart (Db; boxed in Da). Note that TUNEL-positive cells are localized exclusively at the right ventricle of the Smg GDS+/− heart, whereas TUNEL-positive cells are found in both ventricles of the Smg GDS−/− heart. (Dc and Dd) Middle-power (100×; Dc) and high-power (200×; Dd) magnification of the left ventricular wall (boxed in Da) of the Smg GDS−/− heart. Numerous apoptotic cardiomyocytes were readily detected in the endocardium of the Smg GDS−/− heart, whereas apoptotic cells were not found in the left ventricular wall of the Smg GDS+/− heart. lv and rv, left and right ventricles, respectively.