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Genotyping of live mice arising from Smg GDS heterozygous crosses
| Stage | Genotype of Smg GDS-deficient mice
|
||
|---|---|---|---|
| +/+ (%) | +/− (%) | −/− (%) | |
| E12.5 | 9/43 (21) | 20/43 (47) | 14/43 (32) |
| E13.5 | 19/65 (29) | 26/65 (40) | 20/65 (31) |
| E18.5 | 19/64 (30) | 29/64 (45) | 16/64 (25) |
| P0–P7 | 44/143 (31) | 89/143 (62) | 10/143 (7) |
| P21 | 75/270 (28) | 177/270 (66) | 18/270 (7) |
Genotype of Smg GDS-deficient mice is indicated as +/+, +/−, and −/−. Genotyping was performed by Southern hybridization or PCR analysis at the ages indicated.
