FIG. 6.

Binding of CP2 and GATA-1 to erythroid gene regulatory elements. (A) ChIP with anti-GATA-1 or anti-CP2 antibody. Chromatin from K562 cells was immunoprecipitated using antiserum to CP2 or GATA-1. Normal rabbit serum (Ctrl IgG) samples and samples without antibody (No Ab) served as the controls. Quantitative PCR was performed with primer pairs to amplify the erythroid gene regulatory elements (GATA-1 enhancer, p45 NF-E2 promoter, and EKLF enhancer) or the MyoD gene as a control. The input chromatin is shown. (B) ChIP/Re-ChIP with anti-GATA-1 and anti-CP2 antibodies. Chromatin from K562 cells was sequentially immunoprecipitated with anti-CP2 and then anti-GATA-1 antibodies prior to quantitative PCR with the erythroid gene regulatory elements shown. Controls were as described for panel A.