A and B, Single-channel recordings of NSCCs in
outside-out configuration at voltages indicated adjacent to traces.
External solution contained 50 mm NaCl and either 5
mm CaCl2 (A) or 0.5 mm
CaCl2 (B). C, I/V relations of unitary NSCCs (outside: 50
mm NaCl) at 0.5 (triangles) and 5 (circles) mm
CaCl2 with unitary conductances of 10.5 and 7.5
pS, respectively. Data were collected from five independent recordings.
D, Dependence of open probability on membrane voltage (5 mm
CaCl2, 100 mm NaCl); data were
obtained from three independent recordings.