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Light (A) and fluorescent (B) micrographs of a protoplast preparation isolated from seed coats of bean pretreated with the impermeant sulfhydryl reagent, bromobimane. The bromobimane binds specifically to the plasma membranes of ground parenchyma cells responsible for nutrient efflux from the seed coats (Wang et al., 1995). The protoplasts labeled with the fluorescent tag (B) are characterized by large vacuole that is located off center to give a “thumbnail” appearance. Bar = 50 μm.
