FIG. 1.

Us2 interacts with ERK1 and ERK2 in a yeast two-hybrid assay. Verification of protein-protein interactions between Us2 and ERK1 (A) or ERK2 (B). Only yeast strains cotransformed with Us2 and ERK1/ERK2 expression plasmids were able to grow on medium lacking adenine (−Ade) and histidine (−His), indicating a two-hybrid interaction. The ability to grow in the absence of tryptophan (−Trp) indicates selection for the Us2 plasmid. The ability to grow in the absence of leucine (−Leu) indicates selection for the ERK1 and ERK2 plasmids.