TABLE 2.
Susceptibilities of Q151 RT mutants to substrate analogs
| Virus | EC50 (μM)a
|
||||||
|---|---|---|---|---|---|---|---|
| 3TC | AZT | d4T | ddI | PMPA | ABC | PFA | |
| Wild type | 0.90 ± 0.1 (1) | 0.17 ± 0.02 (1) | 5.8 ± 0.9 (1) | 3.1 ± 0.8 (1) | 5.9 ± 1 (1) | 7.0 ± 1 (1) | 92 ± 6 (1) |
| Q151G | 0.79 ± 0.3 (1) | 0.0088 ± 0.001 (0.1) | 0.36 ± 0.1 (0.1) | 1.2 ± 0.3 (0.4) | 0.92 ± 0.1 (0.2) | 1.3 ± 0.1 (0.2) | 93 ± 36 (1) |
| Q151A | 3.4 ± 0.5 (4) | 0.013 ± 0.003 (0.1) | 2.8 ± 2 (0.5) | 1.1 ± 0.1 (0.3) | 1.8 ± 0.2 (0.3) | 2.0 ± 0.5 (0.3) | 62 ± 8 (0.7) |
| Q151V | 9.3 ± 1 (10) | 0.0028 ± 0.001 (0.02) | 0.58 ± 0.2 (0.1) | 1.4 ± 0.1 (0.4) | 0.36 ± 0.1 (0.1) | 3.4 ± 0.5 (0.5) | 6.7 ± 0.9 (0.1) |
| Q151I | 7.7 ± 2 (9) | 0.016 ± 0.001 (0.1) | 0.55 ± 0.04 (0.1) | 0.69 ± 0.3 (0.2) | 0.48 ± 0.1 (0.1) | 3.2 ± 0.5 (0.5) | 11 ± 2.2 (0.1) |
| Q151C | 1.5 ± 0.6 (2) | 0.012 ± 0.002 (0.1) | 0.30 ± 0.05 (0.1) | 0.73 ± 0.2 (0.2) | 0.46 ± 0.1 (0.1) | 0.71 ± 0.2 (0.1) | 150 ± 130 (2) |
| Q151M | 1.4 ± 0.3 (2) | 0.64 ± 0.02 (4) | 23 ± 5 (4) | 11 ± 2 (3) | 7.8 ± 2 (1) | 17 ± 3 (2) | 160 ± 60 (2) |
| Q151M+4b | 6.3 ± 2 (7) | >10 (>100) | >100 (>20) | 39 ± 5 (13) | 18 ± 1 (3) | 56 ± 18 (8) | NDc |
a
EC50s were obtained for HeLa-P4 cells as described in Materials and Methods. Numbers in parentheses indicate EC50s relative to that of the wild-type virus. Values are the means ± standard errors from three or more independent experiments.
c
ND, not determined.