FIG. 5.

Subcellular distribution of BFDV Rep in the absence and presence of CP. Insect cells were infected with recombinant baculovirus and probed with monoclonal antibodies directed against the His₆ affinity tag fused to the N terminus of Rep and CAT. Monoclonal antibody probing was followed by a fluorescein isothiocyanate (FITC)-conjugated secondary antibody (green). DAPI stain was used to define nuclei (blue), while membranes were visualized with Evans blue (red). Merged images (overlay) are also shown.