FIG. 6.

Immunoprecipitation of BFDV Rep and CP expressed in insect cells. Proteins were immunoprecipitated with Tetra-His mouse monoclonal IgG1. Samples were analyzed on a 12% SDS-polyacrylamide gel followed by Western blotting with (a) monoclonal antibodies directed against the His₆ affinity tag fused to the N terminus of Rep or (b) sera from BFDV-infected psittacines. Lanes 1, mock-infected cells; lanes 2, BFDV CP; lanes 3, Rep; lanes 4, Rep plus CP; lanes 5, CAT; lanes 6, CAT plus CP; lanes M, molecular size markers.