FIG. 1.

Identification of Rep-interacting proteins. Flag-immunoprecipitated material from Flag-Rep68-transfected and mock-transfected HEK293 cells was subjected to 10% SDS-PAGE, followed by zinc staining. Protein bands present exclusively in the sample transfected with Flag-Rep68 are marked by asterisks and named after their apparent molecular masses in the gel. The enlarged box shows the presence of a specific band migrating at 31 kDa (p31). These bands were excised from the gel and identified by electrospray tandem mass spectrometry (see text).