FIG. 1.
Genomic maps of replication-defective HSV-2 strains used in this study. All viruses were derived from the wild-type HSV-2 186 strain. The dl5 or dl29 replication-defective mutant virus contains a deletion of the entire UL5 or UL29 gene, respectively. The 5BlacZ mutant virus expresses a nonfunctional ICP8 protein (UL29 gene) fused in frame to the E. coli β-galactosidase (βgal) protein. Viruses were propagated on a complementing Vero-derived cell line, V5-29, which expresses the UL5 and UL29 products upon HSV infection.