Skip to main content
. 2006 Jul;80(14):6964–6972. doi: 10.1128/JVI.00024-06

FIG. 3.

FIG. 3.

Clathrin-mediated entry of HCVcc. (A to C) Huh-7 cells were treated with CHC or control siRNA and then infected with HCVcc, a recombinant Sindbis virus expressing HCV E1, or SV40. (A) Cells were lysed at 5 hpi (Sindbis) or 30 hpi (HCVcc and SV40), and the cell lysates were analyzed by immunoblotting with antibodies to E2, NS3 (HCVcc), E1 (Sindbis), T-antigen (SV40), CHC, and actin. (B) Cells were fixed and processed for immunofluorescent detection of E2 (HCVcc), E1 (Sindbis), or T antigen (SV40). (B) For each virus, the fields presented contain similar numbers of CHC or control siRNA-treated cells. (C) Percentage of infected cells in CHC and control siRNA-treated cells. (D and E) Huh-7 cells were pretreated for 30 min and then infected for 2 h with HCVcc in the presence or the absence of chlorpromazine (CPZ; 5 μg/ml). Cells infected in the absence of drug were cultured with no drug, or chlorpromazine was added 2 hpi (CPZ 2hpi). Infection was analyzed (D) by immunoblotting with antibodies to E2, NS3, GFP (SeV), and actin or (E) by immunofluorescent detection of infected cells using an anti-E2 MAb (HCVcc) or by GFP fluorescence (SeV). Results are presented as percentages of infected cells.