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Effect of expression of N- and C-terminal TBP truncations on the activity of the I1L and I2L promoters. HeLa cells were infected with vaccinia virus and transfected with 2 μg reporter vector DNA driven by the I1L (panel A) or I2L (panel B) promoter and cotransfected with 3 μg N-terminal TBP residues 1 to 159, C-terminal TBP residues 160 to 338, or empty expression vector.
