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. 2006 Jul;80(14):7089–7099. doi: 10.1128/JVI.02694-05

FIG. 4.

FIG. 4.

Protein and nucleic acid content of assembled M-PMV ΔProCANC in sucrose gradient fractions. Average values from five independent measurements are shown. Fraction 11 represents aggregated (pelleted) material. Error bars indicate standard deviations. ΔProCANC M-PMV protein (60 μg) was assembled in the presence of DNA oligonucleotides or RNA in the ratios 60:1 and 10:1 (wt/wt), respectively. The reaction mixtures were centrifuged to equilibrium in a 10 to 60% (wt/wt) sucrose gradient, and amounts of protein and DNA were quantified (see Materials and Methods). The sucrose densities of the fractions were as follows: 1, 0.058 g/ml; 2, 0.069 g/ml; 3, 0.084 g/ml; 4, 1.10 g/ml; 5, 1.12 g/ml; 6, 1.15 g/ml; 7, 1.18 g/ml; 8, 1.21 g/ml; 9, 1.23 g/ml; 10, 1.26 g/ml; and 11, 1.28 g/ml. Shown are (A) RNA of bacteriophage MS2; (B) 16S plus 32S rRNA of E. coli; (C) GT8; (D) GT18; (E) GT22; (F) GTAC22; and (G) ΔProCANC M-PMV protein assembled in the absence of nucleic acids. Grey bars, protein; white bars, DNA oligonucleotides or RNA.