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. 2006 Jul;80(14):7089–7099. doi: 10.1128/JVI.02694-05

FIG. 5.

FIG. 5.

Transmission EM images of negatively stained M-PMV particles assembled from ΔProCANC protein in the presence of GT8, GT18, GT22, and GTAC22 oligonucleotides in the ratio 60:1 (wt/wt). Particles assembled from ΔProCANC protein and GT8 (A), GT18 (B), GT22 (C), and GTAC22 (D). All assembly reactions were performed as described in Materials and Methods. Most of the particles were not fully closed, sometimes having more layers. The proportion of regularly shaped particles sequentially increased in the order of GT8, GTAC22, GT18, GT22 (A, D, B, and C, respectively). The average diameter of assembled particles (in all panels) was 75 nm ± 5 nm. Approximately 20%, 30%, 35%, and 20% of protein was assembled into particles in the presence of GT8, GT18, GT22, and GTAC22 oligonucleotides, respectively. All assembly reactions were performed as mentioned in Materials and Methods. Bar, 100 nm.