FIG. 6.

SDS-PAGE analysis of assembled HIV-1 proteins in sucrose gradient fractions. ΔProCANC and CANC HIV proteins (concentration of 0.6 mg/ml, i.e., 19 μM) were assembled without addition of nucleic acids or with MS2 phage RNA at a ratio of 10:1 (wt/wt). The reaction mixtures were centrifuged to equilibrium through a 10 to 60% (wt/wt) sucrose gradient, and the fractions were analyzed by SDS-PAGE. Fraction 12 represents aggregated material that was pelleted at the bottom of the tube. The sucrose densities of the fractions were as follows: 1, 0.058 g/ml; 2, 0.069 g/ml; 3, 0.084 g/ml; 4, 1.10 g/ml; 5, 1.12 g/ml; 6, 1.15 g/ml; 7, 1.18 g/ml; 8, 1.21 g/ml; 9, 1.23 g/ml; 10, 1.26 g/ml; and 11, 1.28 g/ml. NA, nucleic acid. (A) CANC HIV-1 protein; (B) ΔProCANC HIV-1 protein.