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. 2006 Jul;80(14):6936–6942. doi: 10.1128/JVI.00243-06

FIG. 1.

FIG. 1.

Molecular size exclusion chromatography. (A) HEK-293 and HTB-14 S10 lysates were applied to a Sepharose matrix under constant pressure. After collection of void volume, 1-ml fractions were collected and protein content was analyzed by SDS-PAGE and Western blotting using α-RHA, α-DRBP76, and α-NF45 antibodies. The column was calibrated with known standards of various molecular sizes, as indicated by arrows. Asterisks denote ILF3, a larger isoform of DRBP76. (B) Coimmunoprecipitation of DRBP76 and NF45 from HEK-293 cells. Immunoprecipitates (IP) were generated with α-DRBP76, α-NF45, and mouse immunoglobulin G (IgG), as indicated. Coprecipitating proteins were analyzed by SDS-PAGE and Western blotting with α-DRBP76 and α-NF45 antibodies.