FIG. 2.
Binding patterns of V3-fusion proteins with anti-V3 MAbs derived from individuals infected with non-B-subtype (panels A to D) and subtype B (panels E to H) viruses. Seven human anti-V3non-B MAbs generated from the cells of individuals infected with non-B-subtype viruses (A to D) and nine human anti-V3B MAbs from the cells of subtype B virus-infected subjects (E to H) were tested for their abilities to bind to V3-fusion proteins containing a subtype A V3 sequence (V3A-FP) (A, C, E, G) and to a subtype B V3 sequence (V3B-FP) (B, D, F, H). Human anti-parvovirus B19 MAb 1418 was used as negative control. The curves represent the mean binding activities from three separate experiments, and error bars indicate the standard deviations. The binding curves for anti-V3non-B MAbs are shown in red, with the exception of the curves generated with MAb 2182, which are shown in black (C and D); curves generated with anti-V3B MAbs are shown in blue.