Figure 2.

Ectopic expression of PSY1 in tobacco leads to severe phenotypic effects. A, Leaf of line PSY1⁺10 showing abnormal pigmentation and curling of the leaves. B, Buds of line PSY1⁺32 arrested in flower development. C, White flowers of lines PSY1⁺27, 29, and 31 (right, bottom to top) in comparison with flower of control plant (left). D, Orange capsules of line PSY1⁺27 in comparison with mounted capsule of control plant (red arrow). E, Seedlings from selfing line PSY1⁺31 developing on LS medium containing kanamycin segregate into different phenotypes (wild type, type G [green], type O [orange primary leaves]), aged 2 weeks. F, Homozygous seedlings of line PSY1⁺18 (F₂ generation). G, Dwarf phenotype of primary transformant PSY1⁺10 7 weeks after setting to the greenhouse (left) in comparison with control plant with empty transformation vector (right). H, F₁ generation of line PSY1⁺18, aged 7 weeks, segregating into type G (middle) and type O (right) plants in comparison with control plant (left).