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. 2006 Jun;26(11):4201–4213. doi: 10.1128/MCB.01612-05

FIG. 5.

FIG. 5.

Expression of FRNK does not inhibit ERK signaling but does prevent serum-induced degradation of the CDKIs p27 and p21. (A) Serum-starved HUVECs were transduced with Ad.GFP, Ad.FRNK, or Ad.FRNKS and then stimulated with complete growth medium for 16 h and probed with antibodies specific for pJNK, pERK, pElk, and cyclin D1. (B) Serum-starved HUVECs were transduced with Ad.GFP or Ad.GFP-FRNK prior to stimulation with complete growth medium for 16 h. Cell lysates were made after 16 h and probed with antibodies specific for FAK Y397, p27, p21, pRb S795, and ERK2.