Figure 2.

Functional complementation of CGS-deficient E. coli mutant LE392 with Arabidopsis CGS cDNAs. The plasmid pQE30 (as control), the same plasmid containing the full-length Arabidopsis CGS, and the plasmid containing the truncated version (without the N-terminal region) of CGS were transformed into the E. coli mutant. The transformed bacteria were plated onto an M9 minimal medium with (right) or without (left) Met (40 μg mL⁻¹). Plates were incubated at 37°C for 36 h.