Figure 5.

A, Met; B, SMM; C, Met incorporated to proteins level, from wild type (▪); transgenic lines expressing full-length (▧); and truncated Arabidopsis CGS (□). The amounts of Met and SMM were calculated from total free amino acids plus SMM as detected by HPLC and is given in mol% of this total. The Met level incorporated into proteins was calculated from phosphate-buffered saline-soluble proteins that were subjected to amino acid analysis following protein hydrolysis by HPLC. The levels of soluble Met, SMM, and bound Met were determined from the extraction of leaves of plants grown for 7 weeks. The data are presented as the mean ± se of eight individual plants per line, one measurement per plant. Statistically significant differences (P < 0.05) are identified by letters.