FIG. 5.

p14^ARF stabilizes the Tip60 protein. (A) H358 and H1299 cells were cotransfected with pcDNA3.1-HA-tagged Tip60 (HA-Tip60; 1 μg) and pcDNA3.1-HA-tagged actinin 4 (HA-Act 4; 1 μg) as an internal control, in the presence (+) or absence (−) of pcDNA3.1-p14^ARF (3 μg). Tip60 and actinin 4 (Act 4) were detected by Western blotting using anti-HA antibody. Anti-p14^ARF antibody was used to detect p14^ARF. Actin was used as a loading control. (B) H1299 cells were transfected with pcDNA3.1-HA-Tip60 (1 μg) and pcDNA3.1-HA-actinin 4 (1 μg) in the presence (+p14^ARF) or absence (−p14^ARF) of pcDNA3.1-p14^ARF (3 μg) and treated with CX for the indicated durations. Whole-cell extracts were then subjected to Western blotting using antiactin, anti-HA, and anti-p14^ARF antibodies. Tip60 densitometric signals were normalized to actin as a loading control. A 100% value was arbitrarily assigned to the signal obtained at zero time of cycloheximide treatment. Results are the means ± standard deviations of three independent experiments.