FIG. 1.

Expression of SERP1 in secretory tissue. A. Expression of SERP1 and Sec61 complex in human and mouse secretory tissue. Human endocrine system MTN Blot (I) or mouse MTN Blot II (II) was hybridized with indicated ³²P-labeled cDNA probes. B. Expression of SERP1 and the complex in MIN6 cells. I. MIN6 cells (10⁷ cells/condition) were incubated in medium containing high (H; 25 mM) or low (L; 5.5 mM) glucose for 24 h, and total RNA (10 μg/lane) was used for Northern blotting. II. MIN6 cells (5 × 10⁶ cells/condition) were incubated as described above, and cell extracts were subjected to Western blotting using indicated antibodies. C. Expression of SERP1 and the Sec61 complex in pancreas. Protein extracts of pancreas from C57BL/6 mice after starvation for indicated times were subjected to Western blotting as described above.