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. 2006 Jun;26(11):4257–4267. doi: 10.1128/MCB.02055-05

FIG. 5.

FIG. 5.

Reduced size and enhanced ER stress in the SERP1−/− anterior pituitary. A. Expression of SERP1, Sec61β, and GH in the pituitary. Protein extracts (40 μg) from cerebral cortex (lane 1) or pituitary anterior (lane 2) or posterior (lane 3) lobe of C57BL/6 mice (8 weeks) were subjected to Western blotting with indicated antibodies. B. The size of pituitary anterior lobe. I. Pituitary from mice of the indicated genotype (4 to 8 weeks old, coronal section) was stained with hematoxylin and eosin (n = 4), and representative micrographs are shown. Bar, 200 μm. II. Relative size of pituitary anterior lobe in SERP1+/+, total SERP1−/− (SERP1−/−), and dying SERP1−/− (SERP1−/−D) mice. Area occupied by the anterior (a) lobe was divided by the combined area of posterior (p) and intermediate (i) (area of the p + i lobes was designated as 1). Values shown are means ± standard deviations (n = 4). C. Expressions of GH and ACTH in the anterior pituitary. Pituitary from SERP1+/+, living SERP1−/− (SERP1−/−L), and dying SERP1−/− (SERP1−/−D) mice was stained with hematoxylin and eosin (HE) or immunostained with anti-GH and anti-ACTH antibody. D. Expression/activation of molecules associated with ER stress-induced cell death. Pituitary from SERP1+/+ or dying SERP1−/− (SERP1−/−D) mice was immunostained with the indicated antibodies. E. GH biosynthesis/secretion in SERP1 mice of the indicated genotype (8 weeks of age) after insulin stimulation. ITT were performed as described in Fig. 4, and blood GH levels were measured by ELISA at the indicated times. Values shown are means ± standard deviations (n = 4).