FIG. 4.

Bisulfite genomic sequencing analysis of CpG methylation in the transgene array. (A) Distribution of 20 CpG dinucleotides in the CMVm promoter and 5′ untranslated region of the transgene. (B) Percentage of methylated clones for each CpG. Genomic DNA isolated from cells mock transfected with pBluescriptII KS(−) (silent, black bars) or activated by hTet-Off (gray bars) was used for bisulfite sequencing. A total of 20 individual clones were sequenced to reveal the methylation pattern of the 20 CpG sites over the amplified region.