Skip to main content
. 2006 Jun;26(11):4028–4040. doi: 10.1128/MCB.02189-05

FIG. 5.

FIG. 5.

Northern blot analyses of transgene-derived small RNAs. Low-stringency Northern blot hybridization using radiolabeled DNA probes homologous to the lac operator (lanes 1 to 4), TRE (lanes 5 to 8), CMVm promoter (lanes 9 to 12), and CFP (lanes 13 to 16) sequences in the transgene was performed to detect transgene-specific small RNAs. Small RNA fractions isolated from parental BHK cells (7.5 μg/lane for lanes 1, 5, 9, and 13), clone 2 cells (7.5 μg/lane for lanes 2, 6, 10, and 14), or clone 2 cells transfected with pSilencer plasmids (0.5 μg/lane for lanes 3, 4, 7, 8, 11, 12, 15, and 16) were loaded for probing. The closed triangles (lanes 3, 7, 11, and 15) represent transiently expressed siRNAs. The electrophoretic mobilities of the digoxigenin-labeled 22-nt and 25-nt DNA oligonucleotides are indicated. N, pSilencer-negative.