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. 2006 Jun;26(11):4028–4040. doi: 10.1128/MCB.02189-05

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FIG. 8. — Dicer knockdown does not cause deconstruction of the heterochromatic transgenic locus. ECFP-HP1α (B) and ECFP-SUV39H1 (D) localized to the condensed locus (A and C) in Dicer siRNA-transfected cells. Enrichment of endogenous HP1β (F) and HP1γ (H) at the condensed locus (E and F) in Dicer siRNA-transfected cells was detected by immunofluorescence staining. The transgenic locus is marked by EYFP-LacR (A, C, E, and G). Scale bar, 5 μm. (I) ChIP analysis of H3K9me3 in the CMVm promoter region (black bars) and transcribed CFP region (gray bars), in control or Dicer siRNA-transfected cells. The mean values and standard deviations from three independent experiments are shown.