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. 2006 Jun;26(11):4028–4040. doi: 10.1128/MCB.02189-05

FIG. 9.

FIG. 9.

Effects of overexpressed transgene-directed siRNAs on transgene expression. Cells were activated by transfection with phTet-Off, together with the indicated pSilencer plasmids. The relative CFP mRNA levels in cells expressing transgene-directed siRNAs were determined by normalizing the ratios of CFP mRNA to β-actin mRNA against that of control cells cotransfected with pSilencer-negative. The mean values and standard deviations from three independent experiments using the average value of triplicate RT-PCRs are shown.