TABLE 1.
Overexpression of transgene-directed siRNAs does not antagonize chromatin decondensation (Dox−) or facilitate chromatin recondensation (Dox−→Dox+)
pSilencer | Closed locusa
|
|
---|---|---|
Dox−, 24 h | Dox−, 24 h →Dox+, 2.5 h | |
lac operator | 14 | 68 |
TRE | 13 | 68 |
CMV | 12 | 60 |
CFP | 19 | 64 |
Vector | 14 | 63 |
lac operator + TRE + vector | 18 | 70 |
Negative | 16 | 65 |
Data shown are the number of cells with a closed locus (homogeneous and compact) among a total of 100 EYFP-positive cells.